The Anammox reaction was discovered and validated to be mediated by microbes in the early nineties. In 1999, the responsible bacteria were positively identified. These slowly growing microorganisms belong to the order Brocadiales and are affiliated to the Planctomycetes. Cultivation of these bacteria at sufficient biomass density and rate, as well as the lack of pure cultures, has challenged the study of these extraordinary organisms. Membrane reactors have made the cultivation of up to 95% enriched cell suspensions of Anammox bacteria possible.
KUENEN, J.G. KARTAL, B. JETTEN, M.S.M. (n.y): The Discovery of the Anammox Process and Beyond. Pasadena: The Agouron Institute URL [Accessed: 03.06.2019]